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Merck

HMYOMAG-56K

Millipore

MILLIPLEX® Human Myokine Magnetic Bead Panel

The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.

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About This Item

UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.84

品質水準

化学種の反応性

human

メーカー/製品名

Milliplex®

assay range

accuracy: 100%
(Apelin)

accuracy: 92%
(IL-15)

accuracy: 96%
(FSTL-1)

accuracy: 97%
(LIF)

accuracy: 98%
(Oncostatin M (OSM))

sensitivity: 0.9 pg/mL
(MinDC+2SD; IL-6)

sensitivity: 14 pg/mL
(MinDC+2SD; FGF-21)

sensitivity: 259 pg/mL
(MinDC+2SD; Myostatin/GDF8)

sensitivity: 281 pg/mL
(MinDC+2SD; Irisin)

sensitivity: 4 pg/mL
(MinDC+2SD; LIF)

sensitivity: 40 pg/mL
(MinDC+2SD; Fractalkine/CX3CL1)

sensitivity: 5 pg/mL
(MinDC+2SD; IL-15)

sensitivity: 59 pg/mL
(MinDC+2SD; Osteocrin/Musclin)

sensitivity: 6 pg/mL
(MinDC+2SD; Oncostatin M (OSM))

sensitivity: 64 pg/mL
(MinDC+2SD; Apelin)

sensitivity: 7.5 ng/mL
(MinDC+2SD; Osteonectin (SPARC))

sensitivity: 737 pg/mL
(MinDC+2SD; Follistatin-like Protein 1 (FSTL1))

sensitivity: 815 pg/mL
(MinDC+2SD; Erythropoietin (EPO))

sensitivity: 9 pg/mL
(MinDC+2SD; BDNF)

sensitivity: 9.8 pg/mL
(MinDC+2SD; FABP3)

standard curve range: 0.6-2,400 pg/mL
(IL-6)

standard curve range: 10-40,000 pg/mL
(LIF)

standard curve range: 122-500,000 pg/mL
(Apelin)

standard curve range: 2-10,000 pg/mL
(BDNF)

standard curve range: 2-10,000 pg/mL
(IL-15)

standard curve range: 2-6,000 pg/mL
(Oncostatin M (OSM))

standard curve range: 24-100,000 pg/mL
(FABP3)

standard curve range: 244-1,000,000 pg/mL
(Erythropoietin (EPO))

standard curve range: 244-1,000,000 pg/mL
(Irisin)

standard curve range: 488-2,000,000 pg/mL
(Follistatin-like Protein 1 (FSTL1))

standard curve range: 488-2,000,000 pg/mL
(Myostatin/GDF8)

standard curve range: 49-200,000 pg/mL
(Fractalkine/CX3CL1)

standard curve range: 49-200,000 pg/mL
(Osteocrin/Musclin)

standard curve range: 5-20,000 pg/mL
(FGF-21)

standard curve range: 7-30,000 ng/mL
(Osteonectin (SPARC))

inter-assay cv: <15%
intra-assay cv: <10%
(Apelin)

inter-assay cv: <15%
intra-assay cv: <10%
(BDNF)

inter-assay cv: <15%
intra-assay cv: <10%
(Erythropoietin (EPO))

inter-assay cv: <15%
intra-assay cv: <10%
(FABP3)

inter-assay cv: <15%
intra-assay cv: <10%
(FGF-21)

inter-assay cv: <15%
intra-assay cv: <10%
(Follistatin-like Protein 1 (FSTL1))

inter-assay cv: <15%
intra-assay cv: <10%
(Fractalkine/CX3CL1)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-15)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)

inter-assay cv: <15%
intra-assay cv: <10%
(Irisin)

inter-assay cv: <15%
intra-assay cv: <10%
(LIF)

inter-assay cv: <15%
intra-assay cv: <10%
(Myostatin/GDF8)

inter-assay cv: <15%
intra-assay cv: <10%
(Oncostatin M (OSM))

inter-assay cv: <15%
intra-assay cv: <10%
(Osteocrin/Musclin)

inter-assay cv: <20%
intra-assay cv: <10%
(Osteonectin (SPARC))

テクニック

multiplexing: suitable

検出方法

fluorometric (Luminex xMAP)

輸送温度

wet ice

詳細

Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only act in an autocrine and/or paracrine manner in regulation of skeletal muscle growth, but they also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue and for the profound changes found in immune and neuronal systems.

MILLIPLEX® Human Myokine Panel is a15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum,plasma and cell/tissue supernatant samples: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

アプリケーション

  • Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21), Fractalkine (CX3CL1), Follistatin-Like 1 Protein (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
  • Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
  • NOTE: Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF.
  • Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media.
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
  • Research Category: Bone
  • Research Subcategory: Metabolism

特徴および利点

Design your multiplex kit by choosing available analytes within this panel.

その他情報

Please contact Technical Service for linearity of dilution.

法的情報

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

ピクトグラム

Skull and crossbonesHealth hazardCorrosionEnvironment

シグナルワード

Danger

危険有害性の分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2

ターゲットの組織

Respiratory Tract

保管分類コード

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

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PRTR

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Jan Code

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試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Kelly Ann Hutchinson et al.
Frontiers in physiology, 10, 1188-1188 (2019-10-28)
It is recommended that women accumulate 150-min of weekly moderate-intensity physical activity (MPA) when pregnant. Engaging in regular physical activity (PA) confers many health benefits to both the mother and the fetus. However, the molecular mechanisms by which these health
Xi He et al.
Signal transduction and targeted therapy, 6(1), 427-427 (2021-12-18)
Abnormal glucose and lipid metabolism in COVID-19 patients were recently reported with unclear mechanism. In this study, we retrospectively investigated a cohort of COVID-19 patients without pre-existing metabolic-related diseases, and found new-onset insulin resistance, hyperglycemia, and decreased HDL-C in these
Ayla Karine Fortunato et al.
Journal of immunology research, 2018, 9675216-9675216 (2018-07-27)
Strength exercise is a strategy applied in sports and physical training processes. It may induce skeletal muscle hypertrophy. The hypertrophy is dependent on the eccentric muscle actions and on the inflammatory response. Here, we evaluate the physiological, immunological, and inflammatory
Joanna D C C Lima et al.
Journal of cachexia, sarcopenia and muscle, 10(5), 1045-1059 (2019-07-06)
Cachexia is a paraneoplastic syndrome related with poor prognosis. The tumour micro-environment contributes to systemic inflammation and increased oxidative stress as well as to fibrosis. The aim of the present study was to characterise the inflammatory circulating factors and tumour
Javier Diaz-Castro et al.
Frontiers in nutrition, 8, 761213-761213 (2021-11-09)
Practicing exercise is one of the best strategies to promote well-being and quality of life, however physical activity in schoolchildren and adolescents is developed in an unpredictable, intermittent way and in short periods. There are relatively few intervention studies investigating

関連コンテンツ

MILLIPLEX® Human Myokine Panelのようなマルチプレックスマイオカインイムノアッセイにより、研究者は複数の筋分泌因子を同時に測定することができ、関連する疾患についてより多くの知見を得ることができます。

Myokine analysis with multiplex immunoassays, such as the MILLIPLEX® Human Myokine Panel, allow researchers to simultaneously measure multiple muscle-secreted factors, providing more insights into related diseases.

Simplify protein biomarker discovery with the MILLIPLEX® PLEXpedition 115-Plex Multiplex Screening Panel. Effortlessly screen cytokines and other markers with Luminex® technology.

MILLIPLEX®マルチプレックス代謝アッセイなどのマルチプレックス・イムノアッセイは、メタボリックシンドローム研究に重要です。糖尿病や肥満など、関連するさまざまな病態の全体像を把握でき、時間とサンプル量を節約できるためです。

すべて表示

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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