HEXSM-170K

MILLIPLEX^® Human Exosome Characterization Panel

• 別名: EV multiplex kit, Human exosome multiplex kit, Luminex EV panel, Luminex human exosome immunoassay, Millipore human exosome panel, extracellular vesicle multiplex kit
• UNSPSCコード: 12161503
• NACRES: NA.55

特性

• 交差性: human
• 品質水準: 200
• 包装: pkg of 96 wells
• メーカー／製品名: Milliplex^®
• パラメーター: 25 μL sample volume (per well)
• assay range: intra-assay cv: ≤20%; standard curve range: 0.4-1,500 ng/mL; (CD9); standard curve range: 0.5-2,000 ng/mL; (CD81); standard curve range: 0.6-2,500 ng/mL; (Flotillin-1); standard curve range: 0.9-3,500 ng/mL; (Syntenin-1); standard curve range: 122-500,000 pg/mL; (CD63); standard curve range: 24-100,000 pg/mL; (Calreticulin, GAPDH); standard curve range: 49-200,000 pg/mL; (TSG101); inter-assay cv: ≤10%
• テクニック: multiplexing: suitable
• 検出方法: fluorometric (Luminex^® xMAP^®)
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Cells release extracellular vesicles (EV), including exosomes, as part of normal physiological processes including removal of waste and regulation of intercellular communication. Furthermore, increased exosome release has been implicated in the progression of various disease states and other abnormalities such as cancer and neurodegeneration. Exosome identification, classification, and differentiation from other extracellular vesicles is often complicated due to the variability in isolation and preparation protocols, exosome biogenesis, as well as exosome source. Thus, the development of additional exosome characterization tools is beneficial to the exosome research field. Commonly associated exosome-enriched markers include tetraspanins CD9, CD63, and CD81, cargo sorting-associated markers TSG101 and Flotillin-1, and biogenesis marker syntenin-1. Other markers, including Ago2, GAPDH, and Calreticulin, may or may not be found in exosome-enriched samples, but this can vary depending on source, isolation method, and/or EV biogenesis mechanism. The MILLIPLEX^® Human Exosome Characterization panel provides a novel tool for researchers to study exosomal makers and content in lysed exosome samples.

Research category: Cancer

Research sub-category:
Inflammation & Autoimmune Mechanisms
Inflammation
Infectious Diseases
Immune Response
Tumor Markers

特異性

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, except Syntenin-1 and Flotillin-1 antibodies had minimal cross reactivity with GAPDH standard.

アプリケーション

MILLIPLEX^® Human Exosome Characterization Magnetic Bead Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in exosomes isolated from serum, plasma and tissue culture supernatants: Argonaute-2 (Ago2), Calreticulin (CALR), CD9, CD63, CD81, Flotillin-1 (FLOT-1), GAPDH, Syntenin-1 (Syn-1), and TSG101.

• This is an overnight assay.
• Each assay requires 25 µL per well of diluted or neat isolated exosomes.
• Argonaute-2 (Ago2) is a qualitative assay with the MCF7:IGF-1 Cell Lysate included as a control. All other analytes are for quantitative analysis.
• To test the purity of EV preparation, the MILLIPLEX^® Human Apolipoprotein 6-Plex Panel (Cat. No. APOMAG-62K) can be used.
• To asses the functional component of EVs, the MILLIPLEX^® Human Cytokine/Chemokine Growth Factor Panel A (Cat. No. HCYTA-60K), Panel B (HCYTB-60K), and Multi-Species TGFβ Panel (Cat. No. TGFBMAG-64K-03) can be used.
• Following sample collection and preparation, exosomes can now be isolated and lysed according to your lab’s established protocol. We recommend Cell Signaling Lysis Buffer (Cat. No. 43-040).
• Each lab may use its preferred lysis buffer but must predetermine that the assay will not be negatively affected by the lysis buffer.

特徴および利点

This is a configurable kits. Design your multiplex kit by choosing available analytes within this panel. This panel is available for custom bead premixing.

法的情報

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS08,GHS05,GHS07,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302 + H312 + H332,H315,H317,H318,H373,H410
• 注意書き: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• 危険有害性の分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2
• ターゲットの組織: Respiratory Tract
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3