FCMAB191F

Milli-Mark^® Anti-CD27-FITC Antibody, clone M-T271

clone M-T271, Milli-Mark^®, from mouse

• 別名: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: FITC conjugate
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: M-T271, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Milli-Mark^®
• テクニック: flow cytometry: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_001233.1
• UniProtアクセッション番号: P26842
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CD27(939)

詳細

詳細

CD27, a member of the nerve growth factor receptor (NGFR) superfamily, is a type I transmembrane protein consisting of a disulphide-linked 120 kDa homodimer (Bigler, 1988; Sugita, 1992; Morimoto, 1995). CD27 is expressed on mature thymocytes and on the majority of human peripheral blood T cells, on both CD4+ and CD8+ subsets. CD27 is also expressed on activated B cells and a fraction of resting natural killer (NK) cells (Maurer, 1990; Sugita, 1992). Among CD4+ T cells, CD27 is preferentially expressed on unprimed CD4+CD45RA+CD45R0- T cells, while primed CD4+CD45RA-CD45R0+ T cells express low levels of CD27 (Sugita, 1992). During activation, the expression of CD27 is increased on B cells and unprimed T cells, while primed T cells have a relatively low capacity for upregulation of CD27 (Morimoto, 1995; Hintzen, 1994). The activation is also accompanied by the appearance of a 32 kDa soluble form of CD27 (sCD27) found in supernatants of activated lymphocytes (Hintzen, 1994).
CD27 is believed to play a role in T cell activation, since the expression is upregulated during T cell activation (Bigler, 1988; Sugita, 1992; Morimoto, 1995; Maurer, 1990; Hintzen, 1994; Lens, 1995).

特異性

Antibody recognizes Human CD27 antigen.

免疫原

Leukemic peripheral blood T cells from a patient with chronic T cell leukemia (T-CLL)

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
免疫グロブリン及び免疫

This Milli-Mark Anti-CD27-FITC Antibody, clone M-T271 is validated for use in FC for the detection of CD27.

品質

Evaluated by flow cytometry using human PBMCs

ターゲットの説明

120 kDa (Bigler, 1988; Sugita, 1992; Morimoto, 1995)

物理的形状

Protein A purified

Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.

保管および安定性

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

アナリシスノート

Control
PBMCs

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable