CBL1346Z-I

Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free)

clone RMV-7, from rat

• 別名: Integrin alpha-V, Vitronectin receptor subunit alpha, CD51, 1.Integrin alpha-V heavy chain2.Integrin alpha-V light chain, Integrin alphaV
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: RMV-7, monoclonal
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_032428
• UniProtアクセッション番号: P43406
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Itgav(16410)

詳細

詳細

Integrin alphaV, also known as CD51, a member of the integrin family, is a 140 kD protein expressed on activated T cells, polymorphonuclear granulocytes, blastocysts, and osteoclasts. Integrin alphaV forms heterodimers by association with integrins beta1, beta3, beta5 or beta6, these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The alphaV integrins have varied functions in development, stimulation/activation and homeostasis. The primary ligands for Integrin alphaV complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31.

特異性

Specifically recognizes Integrin alphaV, also known as CD51 or vitronectin receptor. This antibody has been reported to block binding of Integrin alphaV to vitronectin, fibronectin, fibrinogen, and CD31 in some cell types, as well as, blocking LAK cell cytotoxicity.

免疫原

BALB/c mouse IL-2–activated killer (LAK) cells

アプリケーション

This Anti-mouse Integrin alphaV Antibody, clone RMV-7 (Preservative free) is validated for use in Western Blotting, Immunoprecipitation, Flow Cytometry for the detection of mouse Integrin alphaV.

Western Blot Analysis: A representative lot was used by an independent laboratory in LAK cell lysate. (Takahashi, K. et al., (1990). J. Immunol. 145:4371.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in LAK cell lysate. (Takahashi, K. et al., (1990). J. Immunol. 145:4371.)

Blocking Assay: Adhesion and LAK cell cytotoxicity was shown using a representative lot (Takahashi, K. et al., (1990). J. Immunol. 145:4371.)

品質

Evaluated by Flow Cytometry in murine 3T3-L1 cells.

Flow Cytometry Analysis: 0.2 µg of this antibody detected Integrin alphaV in murine 3T3-L1 cells .

ターゲットの説明

~140 kDa observed. The calculated molecular weight is 115 kDa, however Integrin alphaV has been shown as a ~140 kDa band in western blots (Takahashi, K. et al., (1990). J. Immunol. 145:4371.).

関連事項

Replaces: CBL1346Z

物理的形状

Format: Purified

アナリシスノート

Control
Murine 3T3-L1 cells

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable