ABN1739M

Anti-Utrophin A

from rabbit

• 別名: UTRN A
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human, mouse
• 包装: antibody small pack of 25 μg
• テクニック: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_035812
• UniProtアクセッション番号: E9Q6R7
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... UTRN(7402)

詳細

詳細

Utrophin A (UniProt: E9Q6R7) is encoded by the Utrn gene in murine species. Utrophin is a large cytoskeletal protein, which is autosomally-encoded homologue of dystrophin, the protein product of the Duchenne muscular dystrophy (DMD) gene. It displays a sequence homology with dystrophin and possesses many of the protein-binding properties ascribed to dystrophin. In normal skeletal muscle, utrophin is located at the neuromuscular junction and dystrophin predominates at the sarcolemma. During development and in certain myopathies utrophin is also reported to be present at the sarcolemma and this redistribution is associated with higher levels of utrophin. Utrophin is found to co-localize with the acetylcholine receptors at the neuromuscular junctions and may participate in stabilizing the synaptic cytoskeleton. The utrophin mRNA contains two full-length species (named A- and B-utrophin), which are transcribed from different promoters. Hence, utrophin is a composite of A- and B-utrophin and only the A-utrophin is up-regulated in dystrophin-deficient striated muscle. This up-regulation is reported to occur post-transcriptionally. Utrophin A and B differ in their N-termini sequence. (Ref.: Blake, DJ et al. (1996). Brain Pathol. 6(1); 37-47; Weir, AP et al. (2002). J. Biol. Chem. 277 (47); 45285-90).

特異性

This rabbit polyclonal antibody detects utrophin in human and mouse muscle tissue. It targets an epitope with in the first 26 amino acids from the N-terminal region.

免疫原

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the first 26 amino acids from the N-terminal region of murine utrophin.

アプリケーション

Research Category
ニューロサイエンス

Anti-Utrophin A Antibody, Cat. No. ABN1739, is a highly specific rabbit polyclonal antibody that targets Utrophin and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Utrophin A in human skeletal muscle and human uterus tissue.

Immunofluorescence Analysis: A representative lot detected Utrophin A in quadriceps transverse sections in transgenic mice (Adams, M.E., et. al. (2010). J Neurosci. 30(33):11004-10).

Immunohistochemistry Analysis: A representative lot detected Utrophin A in frozen sections of the gastrocnemius muscles (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).

Western Blotting Analysis: representative lot detected Utrophin A in whole muscle lysates (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).

Western Blotting Analysis: A representative lot detected Utrophin A in muscle membrane extracts from mdx mice (Courtesy of Dr Marvin Adams at University of Washington).

Immunofluorescence Analysis: A representative lot detected Utrophin A in mouse neuromuscular junction (Courtesy of Dr Marvin Adams at University of Washington).

品質

Evaluated by Immunohistochemistry in human prostate.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Utrophin A in human prostate tissue.

ターゲットの説明

394.47 kDa calculated.

物理的形状

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): does not flash
• 引火点(℃): does not flash