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由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
human
テクニック
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
dimethylation (Arg3)
遺伝子情報
human ... HIST2H4B(554313)
詳細
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
免疫原
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4, asymmetrically dimethylated at Arg3.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in HeLa cells.
Dot Blot (Specificity) Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
DyLight is a registered trademark of Thermo Fisher Scientific.
Dot Blot (Specificity) Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
DyLight is a registered trademark of Thermo Fisher Scientific.
This Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H4 (Arg3).
品質
Evaluated by Western Blotting in untreated and CARM1 treated recombinant Histone H4.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected dimethyl Histone H4 (Arg3), Asymmetric in 0.5 µg of CARM1 treated recombinant Histone H4.
Western Blotting Analysis: A 1:50,000 dilution of this antibody detected dimethyl Histone H4 (Arg3), Asymmetric in 0.5 µg of CARM1 treated recombinant Histone H4.
ターゲットの説明
~13 kDa observed
物理的形状
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
ABE441:
試験成績書（COA）
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