Ubiquitin-conjugating enzyme E2 A (EC 6.3.2.19; UniProt P49459; also known as hHR6A, HR6A, RAD6 homolog A, Ubiquitin carrier protein A) is encoded by the UBE2A (also known as MRXS30, MRXSN, RAD6A, UBC2) gene (Gene ID 7319) in human. UBE2A accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2A is involved in transcription regulation by catalyzing the monoubiquitination of histone H2B Lys120 to form H2BK120ub1. H2BK120ub1 serves as a specific signal for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, as well as a prerequisite for H3K4me and H3K79me formation. In vitro, UBE2A catalyzes both Lys11 and Lys48-linked polyubiquitination. UBE2A is also involved in UV-damaged DNA repair and aberrant UBE2A can cause mental retardation syndromic X-linked Nascimento-type (MRXSN).
特異性
Expected to react with all three spliced isoforms.
免疫原
Epitope: Near C-terminus
KLH-conjugated linear peptide corresponding to human Ubiquitin-conjugating enzyme E2 A sequence near the C-terminus.
アプリケーション
Research Category エピジェネティクス及び核内機能分子
Research Sub Category 核受容体
This Anti-Ubiquitin-conjugating enzyme E2 A Antibody is validated for use in Western Blotting for the detection of Ubiquitin-conjugating enzyme E2 A.
品質
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ubiquitin-conjugating enzyme E2 A in 10 µg of HeLa cell lysate.
ターゲットの説明
~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
物理的形状
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
