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由来生物
guinea pig
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
human, rat, mouse
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... UCHL1(7345)
mouse ... Uchl1(22223)
rat ... Uchl1(29545)
特異性
Recognizes Protein Gene Product 9.5 (PGP9.5).
免疫原
A 17 amino acid peptide corresponding to amino acids 175-191 from soluble cytoplasmic human PGP9.5.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経細胞及びグリアマーカー
神経細胞及びグリアマーカー
Anti-Protein Gene Product 9.5 Antibody detects level of Protein Gene Product 9.5 & has been published & validated for use in IH.
Immunohistochemistry: 1:500.
Optimal working dilutions must be determined by end user.
blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Optimal working dilutions must be determined by end user.
blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 4°C for 18-24 hours with AB5898 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
物理的形状
Unpurified
Liquid. Contains 0.05% sodium azide.
保管および安定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
アナリシスノート
Control
Brain tissue
Brain tissue
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB5898:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Rebecca S Hornung et al.
Molecular pain, 18, 17448069211069255-17448069211069255 (2022-01-19)
Orofacial pain disorders are predominately experienced by women. Progesterone, a major ovarian hormone, is neuroprotective and antinociceptive. We recently reported that progesterone attenuates estrogen-exacerbated orofacial pain behaviors, yet it remains unclear what anatomical substrate underlies progesterone's activity in the trigeminal
Allison Doyle Brackley et al.
The Journal of biological chemistry, 292(21), 8762-8772 (2017-04-07)
μ-Opioid receptor (MOR) agonists are often used to treat severe pain but can result in adverse side effects. To circumvent systemic side effects, targeting peripheral opioid receptors is an attractive alternative treatment for severe pain. Activation of the δ-opioid receptor
Enhanced insulin clearance in mice lacking TRPM8 channels.
McCoy, DD; Zhou, L; Nguyen, AK; Watts, AG; Donovan, CM; McKemy, DD
American Journal of Physiology. Endocrinology and Metabolism null
M Bagyánszki et al.
Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 23(6), e237-e248 (2011-04-08)
There are indications that alterations in the nitric oxide (NO) system of relaxation mediate gastrointestinal motor disturbances induced by chronic alcohol consumption (CAC). As CAC is known to inhibit the motility of the mouse small intestine, we investigated in this
Nociceptin effect on intestinal motility depends on opioid-receptor like-1 receptors and nitric oxide synthase co-localization.
Sibaev, A; Fichna, J; Saur, D; Yuece, B; Timmermans, JP; Storr, M
World journal of gastrointestinal pharmacology and therapeutics null
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