おすすめの製品
詳細
Microtubule-associated protein 2 (UniProt: P15146; also known as MAP-2) is encoded by the Map2 (also known as Mtap2) gene (Gene ID: 25595) in rat. MAP-2 belong to the family of thermostable proteins associated with microtubules. It is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules against depolymerization. It also has a stiffening effect on microtubules. MAP-2 is shown to be essential for the development and maintenance of neuronal morphology. Three primary isoforms of MAP-2, the high molecular weight MAP2a and MAP2b, and the low molecular weight MAP2c (resulting from alternate splicing of the MAP2 gene) have been identified in neurons. The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation. All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif. MAP-2 can be phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that causes its detachment from microtubules and results in their disassembly.
特異性
Other homologies: Mouse (94% sequence homology). Human (72% sequence homology).
This antibody recognizes MAP-2.
免疫原
Three KLH-conjugated linear peptides corresponding to the N-terminal and central regions of rat MAP-2 .
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経細胞及びグリアマーカー
神経細胞及びグリアマーカー
Anti-MAP-2 Antibody, Cat. No. AB2290, is a highly specific rabbit polyclonal antibody that targets rat MAP-2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in E18 rat hippocampus neurons.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in mouse cerebellum, rat cerebral cortex, and rat kidney tissues.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in mouse cerebellum, rat cerebral cortex, and rat kidney tissues.
品質
Evaluated by Western Blotting in rat brain microsomal preparation.
Western Blotting Analysis: 1 µg/mL of this antibody detected MAP-2 in 10 µg of rat brain microsomal preparation.
Western Blotting Analysis: 1 µg/mL of this antibody detected MAP-2 in 10 µg of rat brain microsomal preparation.
ターゲットの説明
~270 kDa observed; 202.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
Rat brain microsomal tissue lysate
Rat brain microsomal tissue lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 製品選択ツール.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB2290:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Sang Ah Yi et al.
Viruses, 12(9) (2020-09-12)
Since the global outbreak of SARS-CoV-2 (COVID-19), infections of diverse human organs along with multiple symptoms continue to be reported. However, the susceptibility of the brain to SARS-CoV-2, and the mechanisms underlying neurological infection are still elusive. Here, we utilized
Jian-Ying Tian et al.
Experimental and therapeutic medicine, 12(2), 683-689 (2016-07-23)
The aim of the present study was to observe the effects of a general extract of Lycium bararum polysaccharides (LBPs) on methylmercury (MeHg)-induced damage in hippocampus neural stem cells (hNSCs). The hippocampal tissues of embryonic day 16 Sprague-Dawley rats were
Tae-Young Lee et al.
Experimental neurobiology, 29(3), 189-206 (2020-07-02)
Neurogenic differentiation 1 (NeuroD1) is a class B basic helix-loop-helix (bHLH) transcription factor and regulates differentiation and survival of neuronal and endocrine cells by means of several protein kinases, including extracellular signal-regulated kinase (ERK). However, the effect of phosphorylation on
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）