539576

PKA, Catalytic Subunit, Bovine Heart

• 別名: cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 由来生物: bovine heart
• 品質水準: 100
• アッセイ: ≥90% (SDS-PAGE)
• 形状: liquid
• 比活性: ≥20,000 units/μg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• テクニック: activity assay: suitable
• 適合性: suitable for molecular biology
• 輸送温度: wet ice
• 保管温度: −70°C

詳細

詳細

Research area: Cell Signaling

Native PKA catalytic subunit purified from bovine heart. Protein Kinase A (PKA) is a serine protein kinase that targets serine and threonine residues.

生物化学的／生理学的作用

Protein kinases are crucial for integrating signaling networks within eukaryotic cells. It plays an essential role in regulating proliferation and fate determination by influencing Hedgehog signaling transduction. Upon binding of cAMP to the PKA regulatory subunits, the catalytic subunit of PKA dissociates from the PKA holoenzyme and proceeds to phosphorylate target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit is capable of activation without the need for cAMP and is well-suited for in vitro studies of PKA phosphorylation with purified substrates or when introduced into intact cells or cell extracts.

包装

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.

物理的形状

In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.

再構成

Following initial thaw, aliquot and freeze (-70°C).

その他情報

Corbin, J.D., et al. 1988. Methods Enzymol159, 74.
Rannels, S.R., et al 1983. Methods Enzymol99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem.66, 253.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable