5.00526

NOX Inhibitor VII, Thr101

• 別名: NOX Inhibitor VII, Thr101, NADPH Oxidase Inhibitor VII, 6-Fluoro-2- o-tolylbenzo[d]isothiazol-3(2H)-one, 6-Fluoro-2-(2-methylphenyl)-1,2-benzothiazol-3(2H)-one
• 実験式（ヒル表記法）: C₁₄H₁₀FNOS
• CAS番号: 727664-79-1
• 分子量: 259.30
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• アッセイ: ≥98% (HPLC)
• 品質水準: 100
• 形状: powder
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: light brown
• 溶解性: DMSO: 50 mg/mL
• 保管温度: 2-8°C

詳細

詳細

A cell-permeable, selenium-to-sulfur substituted Ebselen (Cat. No. 324483) derivative that exhibits similar NOX2 inhibitory activity as Ebselen by blocking p22phox c-terminal PRD (proline-rich domain) and p47phoxbis-SH3 interaction. Although less potent than Ebselen in cell-free p22phox PRD-p47phoxbis-SH3 binding (IC₅₀ = 4 vs. 0.3 µM) and NOX2 activity (IC₅₀ = 4 vs. 0.6 µM) assays, Thr101 is more potent and NOX2-selective than Ebselen in cell-based assays (Thr101 IC₅₀ /Ebselen IC₅₀ = 0.3/0.5, 3/0.15, 8/no inhibition, 8/0.7 µM, respectively, against NOX2, NOX1, NOX4, and NOX5) and exhibits no glutathione peroxidase activity.

A cell-permeable, selenium-to-sulfur substituted Ebselen (Cat. No. 324483) derivative that exhibits similar NOX2 inhibitory activity as Ebselen by blocking p22phox c-terminal PRD (proline-rich domain) and p47phoxbis-SH3 interaction. Although less potent than Ebselen in cell-free p22phox PRD-p47phoxbis-SH3 binding (IC₅₀ = 4 vs. 0.3 µM) and NOX2 activity (IC₅₀ = 4 vs. 0.6 µM) assays, Thr101 is more potent and NOX2-selective than Ebselen in cell-based assays (Thr101 IC₅₀ /Ebselen IC₅₀ = 0.3/0.5, 3/0.15, 8/no inhibition, 8/0.7 µM, respectively, against NOX2, NOX1, NOX4, and NOX5) and exhibits no glutathione peroxidase activity.

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
blocks p22phox c-terminal PRD (proline-rich domain) and p47phoxbis-SH3 interaction

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Smith, S.M.E., et al. 2012. Chem. Biol.19, 752.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable