Hemocyanin, Keyhole Limpet, Megathura crenulata, Glycerol, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
Carrier protein that has many available primary amines to facilitate protein conjugation. Used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation. Protein: ≥10 mg/ml. Sold on the basis of protein content.
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
物理的形状
In 10 mM MgSO₄, 5 mM BES, 50% glycerol, 0.9% sodium chloride, pH 6.5.
調製ノート
Further dilute with PBS or physiological saline. Material can be dialyzed to remove the glycerol or used as is for conjugation with glutaraldehyde, carbodiimide, or mixed anhydrides.KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.
その他情報
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol.63, 37. Shuler, K.R., et al. 1992. J. Immunol. Methods156, 137.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany