Recombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
アプリケーション
Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
警告
Toxicity: Standard Handling (A)
単位の定義
One unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
物理的形状
In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
その他情報
Kierman, U.A., et al. 2004. Proteomincs4, 1825. Prime, S., et al. 1996. J. Chromatogr. A. 720, 263. Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany