233155

Cleland′s Reagent

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

• 別名: Cleland’s Reagent, DTT, Dithiothreitol, Dithiothreitol, DTT
• 実験式（ヒル表記法）: C₄H₁₀O₂S₂
• CAS番号: 3483-12-3
• 分子量: 154.25
• MDL番号: MFCD00004877
• UNSPSCコード: 12161504
• NACRES: NA.25

特性

• 品質水準: 100
• アッセイ: ≥97% (titration)
• 形状: powder
• 反応適合性: reagent type: reductant
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 色: white to off-white
• 溶解性: water: 30 mg/mL; ethanol: soluble
• 微量陽イオン: heavy metals: ≤1 ppm; heavy metals: <1 ppm
• 輸送温度: ambient
• 保管温度: 2-8°C
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
• InChI Key: VHJLVAABSRFDPM-IMJSIDKUSA-N

詳細

詳細

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.

DTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.

Oxidized dithiothreitol: ≤0.5%.

アプリケーション

• Cyclic nucleotide phosphodiesterase in Dictyostelium discoideum: interconversion of two enzyme forms.: Discusses the role of Cleland′s Reagent in differentiating forms of cyclic nucleotide phosphodiesterase, showcasing its utility in enzymatic studies and its broader implications in biochemistry research (Chassy BM, 1972).

包装

Packaged under inert gas

警告

Toxicity: Harmful (C)

再構成

Following reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O′Brien, P.J. 1991. Mol. Pharmacol.40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett.42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem.243, 716.
Cleland, W.W. 1964. Biochemistry3, 480.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H302,H315,H318
• 注意書き: P264 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable