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品質水準
形状
solution
含まれません
preservative
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
protect from light
色
yellow-brown to reddish
輸送温度
ambient
保管温度
2-8°C
InChI
1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3
InChI Key
OXEUETBFKVCRNP-UHFFFAOYSA-N
詳細
Precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques supplied as a 50X concentrate (0.095 mol/l). To be used with AEC Substrate Buffer, Cat. No. 152226
Precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques. Produces a red end product that is soluble in alcohol. When used in immunohistochemistry, aqueous mounting media, and counterstains must be used. Directions included for use in each application.
アプリケーション
Immunoblotting (see comments)
Immunohistochemistry (see comments)
Immunohistochemistry (see comments)
警告
Toxicity: Toxic (F)
再構成
Discard if a red or brown precipitate is evident.
その他情報
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Okano, K., et al. 1992. Rinsho Byori 40, 93.
Krenacs, T., et al. 1990. Histochem. J. 22, 530.
Krenacs, T., et al. 1990. Histochem. J. 22, 530.
Stable at 4°C and 18-26°C. Functionality determined by performing an immunohistologic stain using the final product.
Suggested procedure for use of AEC in immunohistochemistry and immunoblotting:
1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
2.Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H2O2). Mix well. The final pH of this solution will be ~5.0.
3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.
4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.
5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.
6. If tissue sections require a counterstain, Mayer′s Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.
7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
Suggested procedure for use of AEC in immunohistochemistry and immunoblotting:
1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.
2.Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H2O2). Mix well. The final pH of this solution will be ~5.0.
3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.
4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.
5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.
6. If tissue sections require a counterstain, Mayer′s Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.
7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Danger
危険有害性情報
危険有害性の分類
Carc. 1B - Repr. 2
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
PRTR
第一種指定化学物質
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
152224-10ML:
152224-ML:
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