14-840-M

AMPK (α1, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

• 別名: AMPK kinase
• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 100
• リコンビナント: expressed in E. coli
• フォーム: liquid
• シェルフライフ: 6 mo.
• 分子量: M_w 30 kDa (AMPKß1); M_w 38 kDa (AMPKγ1); M_w 64 kDa (AMPKα1)
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable (kinase)
• 溶解性: water: soluble
• GenBankアクセッション番号: AF100763
• NCBIアクセッション番号: NM_002733; NM_006253
• UniProtアクセッション番号: P54619; Q13131; Q9Y478
• 保管温度: −70°C
• 遺伝子情報: human ... PRKAB1(5564)

詳細

詳細

Research area: Cell Signaling

Product Source: Expressed in E. coli cells and activated in vivo.

Protein Target: AMPK (α1, β1, γ1)

Target Sub-Family: CAMK

Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full- length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1. The AMPK α catalytic subunit in mammals is encoded by α1 and α2, two β genes β1 and β2 and three γ subunit genes γ1, γ2, and γ3.[1]

アプリケーション

Research Category
代謝

アポトーシス及び癌

Research Sub Category
糖尿病

肥満

代謝障害

For use in Kinase Assays.

生物化学的／生理学的作用

Under conditions of nutrient scarcity, AMPK functions as a metabolic checkpoint and inhibits cellular growth.[1] Upon activation, AMPK promotes glucose uptake and fatty acid oxidation thereby reducing energy-consuming processes like protein and lipid synthesis.[2] Furthermore, AMPK regulates autophagy and mitophagy.[3] It also facilitates catabolic pathways to generate more ATP as a response to decreased ATP production.[1]

包装

Also available in 250µg size as catalog # 14-840M - Call for pricing and availability.

品質

Routinely evaluated by phosphorylation of substrate peptide

物理的形状

Cobalt-agarose

保管および安定性

6 months at -70°C

その他情報

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法的情報

GenBank is a registered trademark of United States Department of Health and Human Services

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable