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Merck
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主要文書

安全性情報

12-342

Sigma-Aldrich

Rabbit Anti-Sheep IgG Antibody, HRP conjugate

Upstate®, from rabbit

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.46
価格・在庫情報は確認できません。詳細については、Merckをご覧ください。

由来生物

rabbit

品質水準

結合体

peroxidase conjugate

抗体製品の状態

affinity purified immunoglobulin

抗体製品タイプ

secondary antibodies

クローン

polyclonal

交差性

sheep

メーカー/製品名

Upstate®

テクニック

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

詳細

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

アプリケーション

Research Category
二次抗体及びコントロール抗体
Research Sub Category
全免疫グロブリン検出用二次抗体
Rabbit anti-Sheep IgG Antibody, HRP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA, WB, IH.

品質

Routinely evaluated by western blot

物理的形状

0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate

保管および安定性

Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

11 - Combustible Solids

WGK

WGK 3


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

12-342:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Donel A Sequea et al.
Mechanisms of ageing and development, 134(1-2), 60-63 (2012-12-19)
Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence
Naveen Sharma et al.
PloS one, 8(6), e65118-e65118 (2013-06-12)
Calorie restriction (CR) (consuming ~60% of ad libitum, AL, intake) improves whole body insulin sensitivity and enhances insulin-stimulated glucose uptake by isolated skeletal muscles. However, little is known about CR-effects on in vivo glucose uptake and insulin signaling in muscle.
Susannah L McKay et al.
PloS one, 6(1), e16077-e16077 (2011-02-02)
There is mounting evidence to suggest that the synthesis of pre-mRNA transcripts and their subsequent splicing are coordinated events. Previous studies have implicated the mammalian spliceosomal U2 snRNP as having a novel role in stimulating transcriptional elongation in vitro through
Arvin Bansal et al.
Journal of proteomics, 73(10), 2041-2049 (2010-07-06)
Exercise has been shown to improve function of the left ventricle (LV) following myocardial infarction (MI). The mechanisms to explain this benefit have not been fully delineated, but may involve improved mechanics resulting in unloading effects and increased endothelial nitric
Haiyan Wang et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 310(5), R449-R458 (2016-01-08)
Skeletal muscle insulin resistance is associated with many common age-related diseases, but moderate calorie restriction (CR) can substantially elevate glucose uptake by insulin-stimulated skeletal muscle from both young and old rats. The current study evaluated the isolated epitrochlearis muscle from

質問

1–2/2 質問  
  1. Could you please provide the reconstitution method for this product and the recommended concentration for various uses?

    1 回答
    1. To prepare this product, add 500µl of ultrapure water to make a 1mg/ml stock solution. It is recommended to aliquot the solution to avoid repeated freezing and thawing. The reconstituted solution can be stored for up to 6 months at -20°C. This product is suitable for use in immunohistochemistry at a 1:1000-1:4000 dilution. You can review available Certificates of Analysis by visiting the documentation section on our website.

      役に立ちましたか?

  2. What is the method of reconstitution for this product and what is the concentration recommended for various uses?

    1 回答
    1. This product can be prepared by adding 500µl of ultrapure water to make a 1mg/ml stock solution. Aliquot to avoid repeated freezing and thawing. The reconstituted solution may be stored up to 6 months at -20°C. This product is tested for use in immunohistochemistry, at which a 1:1000-1:4000 dilution is suitable. See the link below to review available Certificates of Analysis: https://www.emdmillipore.com/product/Rabbit-Anti-Sheep-IgG-Antibody-HRP-conjugate,MM_NF-12-342#documentation

      役に立ちましたか?

レビュー

評価値なし

アクティブなフィルタ

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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