1.01301

Auramine O (C.I. 41000)

for microscopy

• 別名: Auramine O (C.I. 41000), Pyoktamin yellow, 4,4′-Bis-(dimethylamino)-benzophenonimide hydrochloride
• 実験式（ヒル表記法）: C₁₇H₂₂ClN₃
• CAS番号: 2465-27-2
• 分子量: 303.83
• MDL番号: MFCD00012484
• UNSPSCコード: 41116130
• EC Index Number: 219-567-2

特性

• 品質水準: 400
• 形状: solid
• 有効性: 1000 mg/kg LD₅₀, oral (Rat)
• pH: 6-7 (20 °C, 10 g/L in H₂O)
• mp: 265 °C (decomposition)
• 溶解性: 10 g/L
• かさ密度: 430 kg/m³
• εmax: 1100-1300 at 431-433 nm at 0.005 g/L (pH 6.0)
• アプリケーション: diagnostic assay manufacturing; hematology; histology
• 保管温度: 2-30°C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• InChI Key: KSCQDDRPFHTIRL-UHFFFAOYSA-N

詳細

詳細

Auramine O (C.I. 41000)-for microscopy, is a dry dye for fluorescence and can be used acc. to Hagemann-Hermannof for the staining acid-fast bacteria (AFB) in smears and sections of human origin . The mechanism of the fluorescence staining is similar to the classical Ziehl-Neelsen method. The cell wall of AFB has a high proportion of wax and lipids and hence absorbs dyes only very slowly. Once the AFB have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, AFB are termed as acid- and alcohol-fast for staining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. Sputofluol^™ also has a disinfectant effect, with the result that any microorganisms that are present are killed off. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

アプリケーション

For microscopy. Staining of mycobacteria according to Hagemann-Hermann, fluorescence staining.
CAS No. 2465-27-2, EC Number 219-567-2.

アナリシスノート

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (buffer pH 6.0)431 - 433 nmSpec. Absorptivity A 1%/1cm (λ_max; 0.005 g/l; buffer pH 6.0; calc. on dried substance)1100 - 1300TLC-Testpasses testLoss on drying (110 °C)≤ 5 %Suitability for microscopypasses test

法的情報

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS07,GHS08,GHS09
• シグナルワード: Warning
• 危険有害性情報: H302 - H319 - H351 - H411
• 注意書き: P201 - P202 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3