1.00327

Hydrogen chloride solution

in ethanol, for microscopy

• 別名: 塩酸エタノール溶液
• 化学式: HCl · CH₃CH₂OH
• CAS番号: 7647-01-0
• 分子量: 36.46
• Beilstein: 1098214
• MDL番号: MFCD00011324
• UNSPSCコード: 41116130
• NACRES: NA.21

特性

• product name: 塩化水素-エタノール 溶液, in ethanol, for microscopy
• 品質水準: 400
• 形状: liquid
• 体外診断薬: for in vitro diagnostic use
• pH: 1.0 (20 °C in H₂O)
• 転移温度: flash point 21 °C
• 密度: 0.90 g/cm³ at 20 °C
• アプリケーション: clinical testing; diagnostic assay manufacturing; hematology; histology
• 保管温度: 2-30°C
• SMILES記法: Cl
• InChI: 1S/ClH/h1H
• InChI Key: VEXZGXHMUGYJMC-UHFFFAOYSA-N

詳細

詳細

Hydrochloric acid in ethanol - for microscopy, is used for human-medical cell diagnosis and serves the purpose of the bacteriological and histological investigation of sample material of human origin. Acid-fast bacteria are difficult to stain because of the high proportion of lipid and wax in their cell walls. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolatefuchsin complex in the cell wall. With the Kinyoun method, a modification of the AFB-Color carbol fuchsin solution renders heating unnecessary (cold staining). Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Product number 1.00327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use malachite green is used for counterstaining.
Pretreatment of the specimens with Sputofluol^™ dissolves the bacteria from the surrounding viscid sputum and cell material. It is an IVD and CE registered product and can be applied for diagnostical purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

アプリケーション

For microscopy. Ziehl-Neelsen staining

法的情報

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS02,GHS05
• シグナルワード: Danger
• 危険有害性情報: H225 - H290 - H319
• 注意書き: P210 - P233 - P234 - P240 - P241 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 1
• 引火点(°F): 69.8 °F
• 引火点(℃): 21 °C