Predicted to cross-react with Mouse and Rat (100% homologous) based on sequence homology.
Recognizes phosphorylated PKCη (Thr655). Not expected to cross-react with other PKC isoforms
免疫原
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PKCη (Thr655).
アプリケーション
Research Category 細胞シグナル伝達
Research Sub Category 細胞骨格シグナル伝達
Immunoblot Analysis: A 0.50 μg/mL dilution of this lot detected phospho-PKCη (Thr655) in RIPA lysate of PMA treated Jurkat cells.
This Anti-phospho-PKCη (Thr655) Antibody is validated for use in WB for the detection of phospho-PKCη (Thr655).
品質
Routinely evaluated by immunoblot.
ターゲットの説明
~80 kDa
物理的形状
Affinity purified
100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide).
保管および安定性
Stable for 2 years at -80°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
A novel function for the binder of Arl two (BART) molecule in pancreatic cancer cells is reported. BART inhibits invasiveness of pancreatic cancer cells through binding to a Ca(2+)-dependent, phosphorylated, guanosine triphosphatase (GTPase) membrane fusion protein, annexin7 (ANX7). A tumor
Journal of biochemistry, 149(3), 331-336 (2010-12-28)
Protein kinase C (PKC) is a family of at least 10 isozymes involved in the activation of different signal transduction pathways. The exact function of these isozymes is not known at present. Isozyme-selective inhibitors would be important to explain the