C2181

抗マウスIgG (全分子) F(ab′)₂ フラグメント–Cy3 ヒツジ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Cy3 Anti-Mouse IgG, Cy3 Mouse IgG, Sheep Anti-Mouse IgG
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD02179333
• Conjugate: CY3 conjugate
• Clone: polyclonal
• Application: IHC (p)
• Citations: 121

特性

• biological source: sheep
• Quality Segment: 200
• conjugate: CY3 conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

詳細

General description

Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.

Application

全てのマウスイムノグロブリンに結合します。
Fcレセプタ-存在下のバックグラウンド染色を避ける際に用いられます。

Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.

Anti-Mouse IgG (whole molecule) F(ab′)₂ fragment−Cy3 antibody produced in sheep has been used:

• in immunolabeling of Hela cells
• as secondary antibody in immunocytochemistry of dendritic cells
• as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
• as secondary antibody in immunofluorescence staining keratinocyte cell lines
• in immunohistochemistry of articular cartilage

Biochem/physiol Actions

Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.

Physical form

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

Preparation Note

ヒトサンプルでのバックグラウンド染色の低減のため吸収処理済み。

Other Notes

本抗体は、ヒト血清タンパク質で吸収済です。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable