07-582-I

Anti-phospho-IRF-3 (Ser396) Antibody

from rabbit, purified by affinity chromatography

• 別名: Interferon regulatory factor 3, IRF-3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• テクニック: western blot: suitable
• NCBIアクセッション番号: NP_001562
• UniProtアクセッション番号: Q14653
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer396)
• 遺伝子情報: human ... IRF3(3661) ; pig ... Irf3(396656) ; rhesus monkey ... Irf3(718970)

詳細

詳細

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

特異性

This antibody recognizes IRF-3 phosphorylated at Ser396.

免疫原

Epitope: SNS → ANA: Mutation of this residue results in loss of viral-induced phosphorylation of IRF-3.

KLH-conjugated linear peptide corresponding to human IRF-3 phosphorylated at Ser396.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
転写因子

Use Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3.

品質

Evaluated by Western Blot in untreated and sendai virus treated A549 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected phospho-IRF-3 (Ser396) in 10 µg of sendai virus treated A549 cell lysate.

ターゲットの説明

~50 kDa observed. Uncharacterized bands may appear at ~40 kDa and ~80 kDa in some lysates.

関連事項

Replaces: 07-582

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Untreated and sendai virus treated A549 cell lysates

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable