07-293-I

Anti-PICK1

from rabbit, purified by affinity chromatography

• 別名: PRKCA-binding protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: rat, human
• 化学種の反応性（ホモロジーによる予測）: mouse (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_032863
• UniProtアクセッション番号: Q62083
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Pick1(18693)

詳細

詳細

PRKCA-binding protein (UniProt Q62083; also known as Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein) is encoded by the Pick1 (also known as Prkcabp) gene (Gene ID 18693) in murine species. PICK1 is a scaffolding protein expressed in many tissues, where it regulates trafficking of multiple membrane proteins. The PDZ (PSD-95/Dlg/ZO-1/DHR (Dlg homologous region) domain (a.a. 22-105) mediates PICK1 interaction with proteins containing PDZ-binding motifs, such as PKC and acid sensing ion channels (ASICs), while the lipid-binding AH (arfaptin homology)/BAR (Bin/amphiphysin/Rvs) domain (a.a. 144-357) mediates PICK1 homo-dimerization and membrane localization. Pick1 gene-disruption results in downregulated ASICs current and calcium transients during process of ischemia-reperfusion and protects against primary murine cortical neurons against injury caused by OGD-Rep (oxygen and glucose deprivation followed by reperfusion). PICK1 is also shown to act as a negatively regulator against ASIC1 phosphorylation/activation by PKA in rat pulmonary arterial smooth muscle cells, where PICK1 facilitates calcineurin-mediated ASIC1 dephosphorylation.

特異性

This rabbit polyclonal antibody targets the C-terminal region of mouse and rat PICK1, as well as human PICK1 spliced isoform 1, but not isoform 2, reported by UniProt (Q62083, Q9EP80, Q9NRD5).

免疫原

Epitope: C-terminus

GST-tagged recombinant mouse PICK1 C-terminal fragment.

アプリケーション

Research Category
ニューロサイエンス

Anti-PICK1, Cat. No. 07-293-I, is a highly specific rabbit polyclonal antibody that targets PRKCA-binding protein and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PICK1 in human cerebellum, kidney, and pancreas tissue sections.

品質

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PICK1 in 10 µg of PC12 rat pheochromocytoma cell lysate.

ターゲットの説明

~55 kDa observed. 46.60 kDa (human/mouse) and 46.61 kDa (rat) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Xia, J., et al. (1999). Neuron. 22(1):179 187).

物理的形状

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable