07-2270

Anti-WNK4 Antibody

from rabbit, purified by affinity chromatography

• 別名: Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 交差性: human, mouse
• 交差性（ホモロジーによる予測）: rat (based on 100% sequence homology)
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBIアクセッション番号: NP_115763
• UniProtアクセッション番号: Q96J92
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... WNK4(65266)

詳細

詳細

WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.

特異性

This antibody recognizes WNK4 at the C-terminus.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human WNK4 at the C-terminus.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
キナーゼ及びホスファターゼ

Immunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected WNK4 in normal human kidney tissue.

This WNK4 antibody is validated for use in WB & IHC for the detection of the WNK4 protein.

品質

Evaluated by Western Blot in mouse kidney cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected WNK4 on 10 µg of mouse kidney cell lysate.

ターゲットの説明

~160 kDa observed. This protein has 3 isoforms produced by alternative splicing. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.

物理的形状

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Mouse kidney cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable