04-804

Anti-NFAT1 Antibody, clone 8G4.2

clone 8G4.2, Upstate^®, from mouse

• 別名: Nuclear factor of activated T-cells, cytoplasmic 2, NF-ATc2, NFATc2, NFAT pre-existing subunit, NF-Atp, T-cell transcription factor NFAT1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 8G4.2, monoclonal
• 交差性: rat, mouse, human
• メーカー／製品名: Upstate^®
• テクニック: immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgMκ
• NCBIアクセッション番号: NP_001129493.1
• UniProtアクセッション番号: Q13469
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NFATC2(4773)

詳細

詳細

NFAT (Nuclear Factor of Activated T-cells) is a family of transcription factors that are located in the cytoplasm in their inactive state. NFAT translocates to the nucleus upon stimulation of the T-cell receptor where it plays a key role in gene expression in immune response. NFAT is dephosphorylated and activated by the phosphatase PP2B/Calcineurin. This results in its nuclear localization and transcriptional activity. For NFAT1 (NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), calcineurin is known to target the region between residues 114-126. Mutations in this region of NFAT1 result in its inability to de-phosphorylate and subsequent nuclear translocation. Upon activation, NFAT mediates the transcriptional induction of cytokines and other immuno-regulatory genes during immune responses, and is expressed in T-cells, B-cells, mast cells and NK cells.

特異性

Detects NFAT1.

Other species have not been tested.

免疫原

Epitope: N-term

GST-fusion protein corresponding to amino acids 1-297 of human NFAT1.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
転写因子

This Anti-NFAT1 Antibody, clone 8G4.2 is validated for use in WB, IF for the detection of NFAT1.

品質

Routinely evaluated by Western Blot

ターゲットの説明

120-140 kDa

物理的形状

Format: Purified

Purified mouse monoclonal in PBS with 0.05% NaN3.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable