Previously, following derivatization to their 2-nitrophenylhydrazide (2-NPH) derivatives, fatty acid (FA) abundances have been evaluated using high-performance liquid chromatography (HPLC). Although the method was sensitive, resolution was insufficient for many of the biologically important FAs. We have developed an enhanced
Clinica chimica acta; international journal of clinical chemistry, 205(1-2), 117-126 (1992-01-31)
An automated assay method has been developed for the measurement of serum cholinesterase activity. The samples were prepared by an automated liquid handling unit and incubated for 9.7 min at 30 degrees C, followed by automatic injection into a colorimetric
Journal of chromatography, 568(1), 25-34 (1991-07-17)
A novel high-performance liquid chromatographic method for biologically important fatty acids incorporated into platelet phospholipids in esterified form has been developed. 2-Nitrophenylhydrazine hydrochloride was used as a pre-column labelling agent to convert the saponified platelet phospholipids directly into corresponding fatty
Journal of the American Society for Mass Spectrometry, 22(11), 1958-1967 (2011-09-29)
Peptides with two or more basic residues, including those with post-translational modifications (PTMs), such as methylation and phosphorylation, can be highly hydrophilic and, therefore, are often difficult to be retained on a reversed-phase (RP) column. In addition, these highly hydrophilic
Journal of AOAC International, 79(2), 493-497 (1996-03-01)
Direct derivatization of saponified fats and oils with 2-nitrophenylhydrazine hydrochloride produced corresponding fatty acid hydrazides without time-consuming sample cleanup and/or extraction steps. Hydrazides were injected directly into a liquid chromatograph. Improved isocratic separation was achieved within only 22 min for
