806536

APN-Maleimide

• 別名: 3-(4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)phenyl)propiolonitrile, MAPN
• 実験式（ヒル表記法）: C₁₃H₆N₂O₂
• 分子量: 222.20
• UNSPSCコード: 12352111
• PubChem Substance ID: 329769017
• NACRES: NA.22

特性

• 形状: powder
• 品質水準: 100
• 保管温度: 2-8°C
• SMILES記法: N#CC#CC1=CC=C(N2C(C=CC2=O)=O)C=C1
• InChI: 1S/C13H6N2O2/c14-9-1-2-10-3-5-11(6-4-10)15-12(16)7-8-13(15)17/h3-8H
• InChI Key: CHKKXKRQICWZFF-UHFFFAOYSA-N

詳細

アプリケーション

APN-Maleimide is a bifunctional crosslinker for thiol-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. Due to kinetic resolution the first thiol reacts exclusively with the maleimide residue producing the protein-APN conjugate. This conjugate can be readily coupled with thiol-containing molecules.

調製ノート

Standard protein labeling procedure (cysteine labeling).

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 10 minutes.
• If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration.
• The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours.

*Note: avoid thiol-containing buffers.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H302,H315,H319,H335
• 注意書き: P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable