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UHPLC Gradient Analysis of Boswellia Herbal Supplement on Ascentis® Express Phenyl-Hexyl

UHPLC Gradient Analysis of Boswellia Herbal Supplement on Ascentis® Express Phenyl-Hexyl application for UHPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53345-U

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

Componente della fase mobile

N° Catalogo
Descrizione
Determinazione del prezzo

452289

Acido fosforico

≥85 wt. % in H2O, ≥99.999% trace metals basis

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 10 cm x 3.0 mm I.D., 2.7 μm particles (53345-U)

mobile phase

[A] 0.1% phosphoric acid; [B] acetonitrile

gradient

50% B for 0.5 min; to 100% B in 4.5 min; held at 100% B for 5 min

gradient

0.6 mL/min

pressure

2390 psi (165 bar)

column temp.

35 °C

detector

UV, 210 nm

injection

2 μL

Descrizione

Applicazioni

extraction:
  1. Weigh 100 mg Bosewellia Herbal Supplement
    2. Add 10 mL ethanol
    3. Sonicate 30 min at 40°C
    4. Dilute 100 μL extract, 400 μL ethanol, 500 μL water

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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