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Discovery® C18 (5 µm) HPLC Columns

L × I.D. 10 cm × 4.6 mm, HPLC Column

Sinonimo/i:

Discovery RP18 HPLC Column

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Colonna HPLC Discovery® C18, 5 μm particle size, L × I.D. 10 cm × 4.6 mm

Materiali

stainless steel column

agenzia

suitable for USP L1

Nome Commerciale

Discovery®

Caratteristiche

endcapped

Produttore/marchio commerciale

Discovery®

Confezionamento

1 ea of

Grado di funzionalizzazione

12% Carbon loading

Parametri

≤70 °C temp. range
400 bar pressure (5801 psi)

tecniche

HPLC: suitable
LC/MS: suitable

Lungh. × D.I.

10 cm × 4.6 mm

Area superficiale

300 m2/g

Copertura della superficie

3 μmol/m2

Impurezze

<10 ppm metals

Matrice

silica gel, high purity, spherical particle platform
fully porous particle

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

5 μm

Dimensione pori

180 Å

operating pH range

2-8

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Descrizione generale

Usare Discovery® C18 per qualsiasi metodo che specifichi una colonna C18. L′eccezionale profilo dei picchi, la riproducibilità e la stabilità ne fanno la colonna di elezione per tutti i metodi C18, da quelli di routine a quelli più critici.

Applicazioni


  • Development of an HPLC-based guanosine monophosphate kinase assay and application to Plasmodium vivax guanylate kinase.: Utilizes the Discovery® C18 HPLC Column for developing a novel enzymatic assay, aiding in malaria research and potential therapeutic target exploration (Pedro et al., 2019).

Caratteristiche e vantaggi

  • Eccellente riproducibilità
  • Picchi eccezionalmente netti per analiti acidi e basici
  • Separazioni LC-MS stabili e a basso spurgo
  • Separazione di peptidi e piccole proteine
  • L′idrofobicità inferiore rispetto a molte colonne C18 confrontabili favorisce analisi più rapide.

Prodotti consigliati

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Note legali

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
Abbas Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(24), 2423-2429 (2011-07-26)
A novel isocratic reversed-phase high performance liquid-chromatography/ultraviolet detection method for simultaneous determination of cefdinir and cefixime in human plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Sample preparation based on a simple extraction
Himal Paudel Chhetri et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 22(5), 483-487 (2014-12-05)
This study presents the optimization of a simple HPLC-UV method for the determination of metformin in human plasma. Ion pair separation followed by UV detection was performed on deproteinized human plasma samples. The separation was carried out on a Discovery
Ramisetti Nageswara Rao et al.
Journal of separation science, 32(9), 1312-1322 (2009-04-29)
Modafinil, adrafinil and their related substances were synthesized and analyzed by RP-LC with ESI-MS/MS. The ionization mode, polarity, cone voltage, and chromatographic conditions were evaluated. The optimum LC-MS conditions to obtain fragment ions indispensable for identification of the structures were
T Nagano et al.
Journal of dental research, 88(9), 823-828 (2009-09-22)
Mmp-20 and Klk4 are the two key enamel proteases. Can both enzymes process amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis? We isolated Mmp-20 and Klk4 from developing pig teeth and used them

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