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W0138

Sigma-Aldrich

Western Blocker™ Solution

for HRP detection systems

Sinonimo/i:

Western blot blocking solution, blocking buffer

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About This Item

Codice UNSPSC:
41105329
NACRES:
NA.25

Livello qualitativo

200

Durata

≥2 yr

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.

Applicazioni

Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.

Caratteristiche e vantaggi

  • Does not inhibit weak HRP signals, as many milk-based blockers do.
  • Can be used both as a blocking component and as a probing component.
  • Supplied ready-to-use; no preparative step is required.

Note legali

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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