SRP3069

IGF-I human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• Sinonimo/i: IGF-IA, Somatamedin C
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 300
• Ricombinante: expressed in E. coli
• Saggio: ≥95% (HPLC)
• Stato: lyophilized powder
• Potenza: 0.1-10 ng/mL ED₅₀/EC₅₀
• PM: ~7.6 kDa
• Confezionamento: pkg of 100 μg; pkg of 50 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: ≤1.00 EU/μg endotoxin, tested
• N° accesso UniProt: P05019
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... IGF1(3479)

Descrizione

Descrizione generale

IGFs (insulin like growth factors) are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin.[1] The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-I expression is regulated by growth hormone.[2] IGF-I signal through the tyrosine kinase type I receptor (IGF-IR).[3] Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.[4] Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and three intra-molecular disulfide bonds.

Applicazioni

IGF-I human has been used:

• as positive control in the quantification of growth factors in adipose-derived stem cells using enzyme-linked immunosorbent assay (ELISA)[5]
• as a medium supplement for trunk crestosphere cultures[6]
• in stimulation protein kinase B (Akt) methylation in human embryonic kidney cells (HEK293) and human melanoma cell line(A375)[7]

In broiler chicks, IGF-I human has been used to study the effect of fadrozole and IGF-1 on female-to-male sex reversal and body weight.[8]

Azioni biochim/fisiol

The IGFs (insulin like growth factors) are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro.[9] IGF1 is an essential component for embryonic and postnatal skeletal development. Absence of IGF1 in mice negatively affects bone development, causes hypomineralization of skeletons and growth plate disturbances.[10] It is also associated with cancer progression. It can suppress cell death and provides resistance to chemotherapy as well as radiation therapy.[11]

Sequenza

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Stato fisico

Lyophilized with no additives.

Ricostituzione

It is recommended to centrifuge the vial prior to reconstitution. A stock solution may be prepared by reconstituting in sterile 0.2% acetic acid (recommended) to a concentration of 1.0 mg/ml. The stock solution may be diluted further using aqueous buffers.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable