SRP0210

PRL-3 (2-173) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Sinonimo/i: PRL-3, PTP4A3, Protein tyrosine phosphatase type IVA, member 3, Protein-tyrosine phosphatase of regenerating liver 3
• Codice UNSPSC: 51111800
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in E. coli
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 19.5 kDa
• Confezionamento: pkg of 50 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_032611
• N° accesso UniProt: O75365
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PTP4A3(11156)

Descrizione

Descrizione generale

Human PRL 3 (GenBank Accession No. NM_032611), (amino acids 2-173) with N-terminal HIS tag, MW = 19.5 kDa, expressed in an E Coli expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definizione di unità

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.