SHC012V
MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles
Contains a gene encoding TagRFP
Sinonimo/i:
MISSION®, MISSION® Control Transduction Particles
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About This Item
Codice UNSPSC:
41106609
NACRES:
NA.51
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Livello qualitativo
Nome Commerciale
MISSION®
Concentrazione
≥1x106 VP/ml (via p24 assay)
tecniche
capture ELISA: 106 TU/mL using p24
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
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Categorie correlate
Descrizione generale
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Applicazioni
MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used to knockdown transient receptor potential vanilloid 1 receptor (TRPV1)-like receptors.[1] It has also been used to generate fluorescent cell lines.[2]
MISSION®pLKO.1-puro-CMV-TagRFP™ Positive Control Transduction Particles has been used in generating fluorescent cell lines by transduction.[2] It has also been used in transfection of human dermal fibroblast (HDF), WI-38 and IMR-90 FBs to express tagRFP.[3]
To see more application data, protocols, vector maps visit sigma.com/shrna.
When conducting experiments using MISSION shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION TagRFP Control Transduction Particles are a positive control to monitor transduction efficiency. The MISSION TagRFP Control Transduction Particles are produced from the sequence-verified 8594 base pair lentivirus plasmid, pLKO.1-puro-CMV-TagRFP (SHC012).
This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest.
This construct aids in interpretation of experimental design and results by providing a red fluorescent protein marker for monitoring success in transduction of your cells of interest.
Note legali
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagRFP is a trademark of Evrogen Co.
Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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