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SAB2702218

Sigma-Aldrich

Monoclonal Anti-6X His tag antibody produced in mouse

clone GT359, affinity isolated antibody

Sinonimo/i:

Mouse Anti-Hexa Histidine tag

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.43

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

primary antibodies

Clone

GT359, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

human, mouse

Concentrazione

1mg/mL

tecniche

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: 500-3000

Isotipo

IgG3

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

Immunogeno

The immunogen used to generate this antibody corresponds to 6X His tag

Applicazioni

Suggested starting dilutions are as follows: ELISA: 1:1000-1:10000, ICC/IF: 1:100-1:2000, IP: 1:100-1:500, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Monoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting.

Azioni biochim/fisiol

6X His tag does not affect the structure and function of protein and can be used in antibody production. It is also used to purify proteins under native or denaturing form.

Caratteristiche e vantaggi

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Altre note

Purification: Affinity purified by Protein G

Stato fisico

Phosphate-buffered saline, no preservative added.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Mohaddeseh Nasir Shirazi et al.
Iranian journal of biotechnology, 22(1), e3602-e3602 (2024-06-03)
Uricase or urate oxidase, as a therapeutic enzyme, is extensively applied to oxidize accumulated uric acid in the body to soluble form to treat related illnesses. This study was conducted with the aim of searching for potential sources of uricase-producing
James S Terry et al.
Virology, 558, 28-37 (2021-03-15)
To help fight COVID-19, new molecular tools specifically targeting critical components of the causative agent of COVID-19, SARS-Coronavirus-2 (SARS-CoV-2), are desperately needed. The SARS-CoV-2 nucleocapsid protein is critical for viral replication, integral to viral particle assembly, and a major diagnostic
Erratum: Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase.
Frontiers Production Office
Frontiers in Bioengineering and Biotechnology, 10, 878674-878674 (2022)
SKP2-and OTUD1-regulated non-proteolytic ubiquitination of YAP promotes YAP nuclear localization and activity.
Yao F, et al.
Nature Communications, 9(1), 2269-2269 (2018)
Jordan Demone et al.
PloS one, 17(12), e0277668-e0277668 (2022-12-15)
Agroinfiltration is a method used in biopharming to support plant-based biosynthesis of therapeutic proteins such as antibodies and viral antigens involved in vaccines. Major advantages of generating proteins in plants is the low cost, massive scalability and the rapid yield

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.