The detection limit of product H1029 is 0.5 μg/ml or less.
H1029
Monoclonal Anti-polyHistidine antibody produced in mouse
clone HIS-1, ascites fluid
Sinonimo/i:
Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag
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100 μL
510,00 €
0.2 ML
655,00 €
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Cambia visualizzazione
100 μL
510,00 €
0.2 ML
655,00 €
About This Item
Codice UNSPSC:
12352203
NACRES:
NA.56
Origine biologica
mouse
Livello qualitativo
Coniugato
unconjugated
Forma dell’anticorpo
ascites fluid
Tipo di anticorpo
primary antibodies
Clone
HIS-1, monoclonal
Stato
liquid
tecniche
dot blot: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
Isotipo
IgG2a
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Descrizione generale
The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.
Specificità
The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Immunogeno
recombinant polyHistidine tagged fusion protein.
Applicazioni
Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.
Azioni biochim/fisiol
The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.
Stato fisico
Suppled as ascites fluid with 15mM sodium azide.
Altre note
Recommended dilution for immunoblotting is 1:3000
Note legali
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
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What is the detection limit of Product H1029, Monoclonal Anti-polyHistidine antibody?
1 risposta-
Utile?
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Is Product H1029, Monoclonal Anti-polyHistidine antibody, suitable for use in immunofluorescence studies?
1 risposta-
Product H1029 has been used in immunofluorescence and a good picture of the staining has been published. Ker R. Marshall, et al., Journal of General Virology 83, 1601-1612 (2002).
Utile?
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How many histidines are required for proper recognition by Product H1029, Monoclonal Anti-polyHistidine antibody?
1 risposta-
Product H1029, Monoclonal Anti-polyHistidine antibody requires at least 6 histidines in a row for recognition.
Utile?
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