S8884

Safranin O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

• Sinonimo/i: Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
• Formula empirica (notazione di Hill): C₂₀H₁₉ClN₄
• Numero CAS: 477-73-6
• Peso molecolare: 350.84
• Colour Index: 50240
• Beilstein: 3924099
• Numero CE: 207-518-8
• Numero MDL: MFCD00011759
• Codice UNSPSC: 12171500
• ID PubChem: 24899820
• NACRES: NA.47

Proprietà

• product name: Safranin O, certified by the Biological Stain Commission
• Grado: certified by the Biological Stain Commission
• Forma fisica: powder
• Composizione: Dye content, ≥80%
• Colore: green to very dark green
• Solubilità: H₂O: 1 mg/mL
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: [Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
• InChI: 1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H; OARRHUQTFTUEOS-UHFFFAOYSA-N

Descrizione

Descrizione generale

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Applicazioni

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Compatibilità

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H318
• Consigli di prudenza: P280 - P305 + P351 + P338
• Classi di pericolo: Eye Dam. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves