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P4689

Sigma-Aldrich

Protein G′ from proprietary source

recombinant, expressed in E. coli, lyophilized powder

Sinonimo/i:

G′ protein

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1 MG
116,00 €
5 MG
395,00 €

116,00 €


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Cambia visualizzazione
1 MG
116,00 €
5 MG
395,00 €

About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

116,00 €


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Ricombinante

expressed in E. coli

Livello qualitativo

Coniugato

unconjugated

Stato

lyophilized powder

Capacità

~5 mg/mg, solid binding capacity (IgG)

Temperatura di conservazione

−20°C

Descrizione generale

Genetically engineered truncated protein G; retains affinity for IgG, but lacks albumin- and Fab- binding sites and membrane-binding regions.[1]
Protein G is a group G Streptococcus protein and is a large multi-domain cell wall protein. It interacts with the Fc region of IgG (immunolglobulin) through its repeating 55-amino acid domain. Strain GX7809 and GX7805 contain two and three such protein repeats respectively.[2][3]

Applicazioni

Protein G has been used for the analysis of mAb (monoclonal antibody) synergy towards hCG (human chorionic gonadotropin) using surface plasmon resonance (SPR), and for serum IgG galactosylation obtained from RA (rheumatoid arthrtitis) patients and MRL-lpr mice.[4][5]

Azioni biochim/fisiol

Protein G is implicated in the evasion of host defence response by Streptococcus, via its protein binding properties. It is interacts with α2-micorglobulin which is a predominant inhibitor of human plasma.[2]

Stato fisico

Lyophilized from water.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Agalactosyl IgG and beta-1,4-galactosyltransferase gene expression in rheumatoid arthritis patients and in the arthritis-prone MRL lpr/lpr mouse.
Jeddi PA et al
Immunology, 87(4), 654-659 (1996)
Haim Ashkenazy et al.
Journal of molecular biology, 433(15), 167071-167071 (2021-05-31)
Antibodies provide a comprehensive record of the encounters with threats and insults to the immune system. The ability to examine the repertoire of antibodies in serum and discover those that best represent "discriminating features" characteristic of various clinical situations, is
Enhancement in antigen binding by a combination of synergy and antibody capture.
Klonisch T et al
Immunology, 89(2), 165-171 (1996)
C R Goward et al.
The Biochemical journal, 267(1), 171-177 (1990-04-01)
The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the albumin-binding domains and the Fab-binding region were then deleted by site-directed mutagenesis. The translation of regions
Arie Ryvkin et al.
PloS one, 7(8), e41469-e41469 (2012-08-08)
Polyclonal serum consists of vast collections of antibodies, products of differentiated B-cells. The spectrum of antibody specificities is dynamic and varies with age, physiology, and exposure to pathological insults. The complete repertoire of antibody specificities in blood, the IgOme, is

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