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Key Documents

OGS590

Sigma-Aldrich

PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID

plasmid vector for molecular cloning

Sinonimo/i:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.85

Forma fisica

buffered aqueous solution

PM

size 2686 bp

Selezione batterica

ampicillin

Origine di replicazione

pUC (500 copies)

Clivaggio proteico

no cleavage

Promotore

Promoter name: Lac
Promoter activity: inducible
Promoter type: bacterial

Gene reporter

none

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

Descrizione generale

This high-copy number plasmid is the versatile industry standard cloning vector that can be used to insert and sub-clone genes using blue/white screening.

Applicazioni

Cloning in a gene: PUC19 - HIGH COPY BLUE/WHITE CLONING PLASMID is a standard cloning plasmid used by thousands of laboratories for routine cloning. The plasmid can be used for blue-white screening and contains a diverse multiple cloning site that extends from the EcoRI to Hind3 restriction sites that allows DNA sequences to be inserted.

Sequenza

To view sequence information for this product, please visit the product page

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.oxgene.com

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
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Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
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Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

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