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N2912

Sigma-Aldrich

Anti-Neurofilament 160/200 antibody, Mouse monoclonal

~2 mg/mL, clone RMdO20, purified from hybridoma cell culture

Sinonimo/i:

Anti-NEF3

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About This Item

Numero MDL:
MFCD00164708
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

200

Coniugato

unconjugated

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

RMdO20, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

mouse, rat, human

Concentrazione

~2 mg/mL

tecniche

immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using extracts of rat brain S1 fraction.

Isotipo

IgG1

N° accesso UniProt

P07197

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NEFH(4744) , NEFM(4741)
mouse ... Nefh(380684) , Nefm(18040)
rat ... Nefh(24587) , Nefm(24588)

Descrizione generale

Neurofilaments are 10 nm in diameter and belong to intermediate filament protein family. Neurofilaments are expressed mainly in cells or tissues of neuronal origin and are crucial for proper radial growth of axons. Monoclonal anti-neurofilament 160/200 antibody can be used in ELISA and immunoblotting. It can also be used in microarray. Mouse anti-neurofilament 160/200 antibody reacts specifically with the non-phosphorylated form of NF-M and NF-H (NF-M/H, approx.160 and 200 kDa) in mouse, rat and human.

Immunogeno

purified mid-size rat neurofilament (NF-M) subunit.

Applicazioni

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Monoclonal anti-neurofilament 160/200 antibody can be used in immunocytochemistry (diluted 1: 100) and western blotting (diluted 1: 1,000) . It can also be used in immunohistochemistry.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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