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MTOX1004

Sigma-Aldrich

MDR1/BCRP Double Knockout Caco-2 Cells

Human male colorectal tissue, adenocarcinoma

Sinonimo/i:

C2BBe1 Cells MDR1/BCRP (-/-/-/-,-/-/-/-)

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About This Item

Codice UNSPSC:
41106514
NACRES:
NA.81
Il prodotto MTOX1004 al momento non è in vendita nel tuo paese Contatta l'Assistenza Tecnica.

Nome del prodotto

MDR1/BCRP Double Knockout Caco-2 Cells, one vial

Origine biologica

human male colorectal tissue (Source disease: adenocarcinoma)

Stato

liquid

tecniche

drug transporter assay: suitable
permeability assay: suitable

N° accesso OMIM

171050
603756

applicazioni

ADME/TOX

Temperatura di conservazione

−196°C

Informazioni sul gene

human ... ABCB1(5243) , ABCG2(9429)

Descrizione generale

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 and ABCG2 (MDR1/BCRP) efflux transporters.

Applicazioni

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Caratteristiche e vantaggi

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
  • A functional double knockout of the MDR1 gene and BCRP gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP2 substrate
  • The vial format enables the MDR1 and BCRP knockout cells to be included in standard drug transporter protocols
  • Human assay with no interference from animal inhibitors
  • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Note legali

ADME/Tox Cell Lines License

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • MTOX1004MDR1/BCRP Double Knockout Caco-2 Cells, one vial 1 vialSDS

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLBW5057
SLBG6734
SLBB2194

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X Wu et al.
Pharmaceutical research, 17(2), 209-215 (2000-04-06)
The purpose of this study was to elucidate the mechanisms by which an HMG-CoA reductase inhibitor, atorvastatin (an organic acid with a pKa of 4.46), was transported in the secretory and absorptive directions across Caco-2 cell monolayers. Caco-2 cells were
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
S Yee
Pharmaceutical research, 14(6), 763-766 (1997-06-01)
To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in man. Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous
P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
Kathleen E Sampson et al.
Drug metabolism and disposition: the biological fate of chemicals, 43(2), 199-207 (2014-11-13)
Membrane transporters P-glycoprotein [P-gp; multidrug resistance 1 (MDR1)], multidrug resistance-associated protein (MRP) 2, and breast cancer resistance protein (BCRP) affect drug absorption and disposition and can also mediate drug-drug interactions leading to safety/toxicity concerns in the clinic. Challenges arise with
Visualizza tutti i documenti correlati

Articoli

The Role of Intestinal Efflux Transporters In Drug Absorption

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

Drug transport assays in a 96-well system: reproducibility and correlation to human absorption

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

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