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MCL1

Sigma-Aldrich

Mammalian Cell Lysis Kit

Sinonimo/i:

Cell Lysis Reagent

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

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1 KIT
613,00 €

613,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.


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Cambia visualizzazione
1 KIT
613,00 €

About This Item

Codice UNSPSC:
12352200
NACRES:
NA.56

613,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

impiego

 kit sufficient for 250 extractions (100 mm tissue culture plates)

Livello qualitativo

tecniche

immunoprecipitation (IP): suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Categorie correlate

Applicazioni

The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.[1]

Caratteristiche e vantaggi

  • The kit can be used to prepare cell lysis buffer and other modified buffers
  • The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein

Componenti

The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • P8340Protease inhibitor cocktail 2.5 mLSDS

Pittogrammi

CorrosionExclamation mark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from
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Frontiers in physiology, 6, 69-69 (2015-03-26)
In many non-excitable and excitable cells, store-operated calcium entry (SOCE) represents an additional pathway for calcium entry upon Ca(2+) store depletion. In a previous study, we demonstrated SOCE activity in intact mouse cardiac pacemaker tissue, specifically from sinoatrial node (SAN)

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