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MAK263

Sigma-Aldrich

Glucose Colorimetric/Fluorometric Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric or fluorometric tests

applicazioni

cosmetics
food and beverages

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

endocrinological disorders, diabetes

Temperatura di conservazione

−20°C

Descrizione generale

Glucose is a primary energy source that naturally occurs in its free state in fruits and other plant parts. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose– related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulphonylureas in type 2 diabetics.

Caratteristiche e vantaggi

Compatible with high-throughput handling systems.

Compatibilità

Suitable for the determination of glucose concentrations in various biological samples, including serum, plasma, food, or growth medium. This kit is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.

Principio

Glucose is oxidized to generate a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the amount of glucose present. The kit is able to detect 1-10,000 μM of glucose in various samples.

Prodotti correlati

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Descrizione
Determinazione del prezzo

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334,H412

Consigli di prudenza

P261 - P273 - P284 - P501

Classi di pericolo

Aquatic Chronic 3 - Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
8J20K06060
8H05K06060
5K30K06060
J20K06060
8F22K06060

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Ryan K Perkins et al.
Nutrients, 11(3) (2019-03-03)
The purpose of this investigation was to evaluate the effects of experimental hyperglycemia on oxidative damage (OX), advanced glycation end products (AGEs), and the receptor for AGEs (RAGE) through an in vivo approach. Obese subjects (n = 10; 31.2 ±
Jake N Barber et al.
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Species loss within a microbial community can increase resource availability and spur adaptive evolution. Environmental shifts that cause species loss or fluctuations in community composition are expected to become more common, so it is important to understand the evolutionary forces
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The red palm weevil (RPW), Rhynchophorus ferrugineus, is one of the worst palm pests worldwide. In this work, we studied the physiological basis underlying its adaptive strategy against low temperatures. Specifically, we analyzed the main low-molecular-weight biochemical substances acting as
Gemma K Kinsella et al.
International journal of molecular sciences, 22(19) (2021-10-14)
GPR21 is a constitutively active, orphan, G-protein-coupled receptor, with in vivo studies suggesting its involvement in the modulation of insulin sensitivity. However, its precise contribution is not fully understood. As the liver is both a major target of insulin signalling
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Domande

1–2 di 2 domande  

  1. Can this kit be used on solid tissue samples? If so, how should the tissue be homogenized?

    1 risposta

    1. This kit can be used for tissue samples. To homogenize, please proceed as follows:
      Homogenize the tissue sample in assay buffer using the following protocol:

      Start with approximately 10 mg of tissue (the amount may need to be optimized), wash in cold PBS and resuspend in ~100 uL of assay buffer provided with the kit.
      Homogenize using a Dounce homogenizer or a pestle, on ice to keep the sample cold (10-15 passes).
      Centrifuge at 4°C in a cold microcentrifuge to remove insoluble.
      Collect the supernatant and keep it on ice throughout sample preparation.

      Use a few different volumes of this supernatant (between 2 and 50 uL) and bring them up to a total volume of 50 µl/well with Glucose Assay Buffer in a 96-well plate, as recommended for a typical liquid sample.
      The reason for trying different volumes of samples is to ensure the readings are within the standard curve range.
      Then proceed with the 'Assay Reaction' step.

      Please see the datasheet below for the assay reaction step:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

      Utile?

  2. We require a protocol for #MAK263 for my customer

    1 risposta

    1. Please see the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/602/mak263bul.pdf

      Utile?

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