The new Isocitrate Assay Kit, MAK524, is now available! Isocitrate is a TCA cycle intermediate that is formed from citrate by the activity of the enzyme aconitase. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. In plants and lower organisms, isocitrate is the branch point from which the glyoxylate shunt operates. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. The Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples.
Compatibilità
Suitable for quantifying isocitrate in a variety of samples including tissue, cells, food and beverages
Principio
Isocitrate concentration is determined by a coupled enzyme assay, which results in a colorometric (450 nm) product, proportional to the isocitrate present. Typical sensitivities of detection for this kit are 1-20 nmoles (0.2 - 5 μg) of isocitrate.
We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.
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