Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf
M9274
Murashige and Skoog Basal Medium
suitable for plant cell culture, with sucrose and agar
Sinonimo/i:
MS Basal Medium
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1 L
13,90 €
10 L
71,70 €
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Cambia visualizzazione
1 L
13,90 €
10 L
71,70 €
About This Item
Prodotti consigliati
Livello qualitativo
Stato
powder
tecniche
cell culture | plant: suitable
applicazioni
agriculture
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Applicazioni
Formula variabile
With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
Quantità
Formulated to contain 42.4 grams of powder per liter of medium.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Ox. Sol. 3
Codice della classe di stoccaggio
5.1B - Oxidizing hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Elenchi normativi
Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XVII (Restriction List)
CAS No.
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I clienti hanno visto anche
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P Panichayupakaranant et al.
Pharmaceutical biology, 48(11), 1192-1197 (2010-09-17)
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Would an incorrect pH level affect the setting of the agar?
1 risposta-
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How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
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This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
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This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
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How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
1 risposta-
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
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