K1757

Keratinocyte Growth Factor human

≥95% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, powder, suitable for cell culture

• Sinonimo/i: hFGF-7, hKGF, FGF-7, KGF
• Numero CAS: 148348-15-6
• Numero MDL: MFCD00212245
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Nome del prodotto: Keratinocyte Growth Factor human, KGF, recombinant, expressed in E. coli, powder, suitable for cell culture
• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Saggio: ≥95% (SDS-PAGE and HPLC)
• Stato: powder
• Potenza: 0.1-15 ng/mL ED₅₀/EC₅₀
• Qualità: endotoxin tested
• PM: 18.9 kDa
• Confezionamento: pkg of 10 and 50 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: 1 EU/μg
• N° accesso UniProt: P21781
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... FGF7(2252)

Descrizione

Descrizione generale

Keratinocyte Growth Factor(KGF) is a 18.9 kDa growth factor, spanning 163 amino acids,[1] belongs to the fibroblast growth factor family. It includes a secretory signal sequence and shares 39% identity with FGF-Basic. It is expressed in a number of stromal fibroblast cell lines[2], mesenchymal cells[3] but absent from normal glial cells and epithelial cell lines.

Applicazioni

Keratinocyte Growth Factor human has been used:

• as a component of epithelial growth medium[4]
• as a positive control for antigen to determine the activity of the antibody[5]
• to analyze the influence of KGF associated with thermo-sterilized carboxymethyl chitosan (NOCCts) in the reduction of pericardial adhesions[6]

Azioni biochim/fisiol

Keratinocyte Growth Factor(KGF) stimulates proliferation and differentiation of keratinocytes and other cells in the epithelium.[7] It induces chemotaxis of microvascular endothelial cells and neovascularization of rat cornea. It stimulates the hyaluronic acid synthesis in proliferating and migrating basal cells.[2] KGF contributes to the survival of cells under stress conditions.[3] KGF is highly expressed in injured and inflamed epithelial cells, thereby, promotes wound healing.[3]

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Risultati analitici

Determined by a cell proliferation assay using 4MBr-5 cells.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves