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HSANGERV

Sigma-Aldrich

Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Human, Virus Format

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About This Item

Codice UNSPSC:
41105904

Livello qualitativo

200

Confezionamento

pkg of 10 μL (384-well plate)

Concentrazione

1x106  VP/ml (via p24 assay)

applicazioni

CRISPR

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

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Categorie correlate

Descrizione generale

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Applicazioni

Functional Genomics/Screening /Target Validation

Caratteristiche e vantaggi

  • Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
  • Simplify the workflow with puromycin selection
  • Illuminate CRISPR-expressing cells with BFP

Additional Features
  • Better, not bigger: Two optimized clones per gene reduces the time, cost, and scale of screening experiments
  • Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
  • Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Confezionamento

384-well plates

Componenti

Lentivirus Transduction Particles of gRNA-only lenti-based vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones.


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Stato fisico

10 μl of lentivirus at ≥106 particles/ml in 102×384-well plates

Altre note

This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Prodotti consigliati

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Mouse, Virus Format

Note legali

CRISPR Use License Agreement

Lentiviral License Agreement

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.
Metzakopian, E. et al.
Scientific Reports, 7 (2017)

Articoli

The Researcher’s Guide to Designing CRISPR and RNAi Screening Experiments

This screening guide covers how to choose a cell line, a screening library, and experimental conditions as well as tips for designing and performing your experiment.

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Protocolli

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Sequenziamento con metodo Sanger, procedura e passaggi

Scopri tutti i passaggi del sequenziamento secondo Sanger, detto anche “metodo di terminazione della catena”, come funziona il sequenziamento del DNA e come leggere accuratamente i risultati per ottimizzare la tua ricerca.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.