GE17-5318-01
Ni Sepharose™ 6Fast Flow
Cytiva 17-5318-01, pack of 25 mL
Sinonimo/i:
Sepharose Fast Flow
About This Item
Prodotti consigliati
Confezionamento
pack of 25 mL
Produttore/marchio commerciale
Cytiva 17-5318-01
Matrice
6% cross-linked agarose
Dimensione particelle
45-165 μm
Diametro medio
90 μm
cleaning in place
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
Capacità
~40 mg binding capacity(histidine-tagged protein)
Compatibilità
suitable for bioprocess medium
Categorie correlate
Descrizione generale
Ni Sepharose™ 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.
Ni Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.
Ni Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Ni Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Caratteristiche e vantaggi
- Designed for scaling up purification of histidine-tagged proteins
- High protein binding capacity and minimal leakage of Ni2? ions
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep™ and HisTrap™ column formats.
Stoccaggio e stabilità
Risultati analitici
Note legali
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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Articoli
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.
Protocolli
HisTrap™ FF Kit purifies histidine-tagged proteins directly from cell lysates, minimizing sample pretreatment for rapid purification.
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from Cytiva.
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
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