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GE17-5318-01

Ni Sepharose 6Fast Flow

Cytiva 17-5318-01, pack of 25 mL

Sinonimo/i:

Sepharose Fast Flow

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

Confezionamento

pack of 25 mL

Produttore/marchio commerciale

Cytiva 17-5318-01

Matrice

6% cross-linked agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning in place

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

Capacità

~40 mg binding capacity(histidine-tagged protein)

Compatibilità

suitable for bioprocess medium

Descrizione generale

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni?? for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged proteins. The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.

Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caratteristiche e vantaggi

  • Designed for scaling up purification of histidine-tagged proteins
  • High protein binding capacity and minimal leakage of Ni2? ions
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pittogrammi

Environment

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Protocolli

HisTrap FF Kit purifies histidine-tagged proteins directly from cell lysates, minimizing sample pretreatment for rapid purification.

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.

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