b.End3

96091929, mouse brain, Endothelial-like

• Sinonimo/i: bEND.3 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: b.End3, 96091929
• Origine biologica: mouse brain
• Disponibilità: available only in EU
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Endothelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse SV129 brain endothelioma

Descrizione della linea cellulare

The cell line b.End3 has been established from brain endothelial cells of SV129 mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End3 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as ICAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.

Applicazioni

Study of endothelial cell growth, differentiation and influence of oncogenes.

Terreno di coltura

DMEM + 2mM Glutamine + 5μM 2-Mercaptoethanol (2ME) + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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