HERLUF human cell line

IHW Number: 9299, 87031104

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human blood
• Etnia: Danish
• Genere: male
• Modalità di accrescimento: Suspension
• Cariotipo: Not specified
• Morfologia: Lymphoblastoid
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice

Descrizione

Descrizione generale

Consanguineous: No

Descrizione della linea cellulare

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profilo DNA

A: 2
Cw: 0501 04
B: 4402 35
HLA DRB1: 1201
HLA DRB3: 202
HLA DQA1: 501
HLA DQB1: 301
HLA DPA1: 01 02
HLA DPB1: 401

Profilo sierologico

HLA A: 2
HLA C: 4 5
HLA B: 44 35
Bw4/bw6: 4 6
HLA Dw: DB6
HLA DR: 12
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 7
HLA DP: 4

Terreno di coltura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Mantenimento delle subcolture

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Altre note

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Esclusione di responsabilità

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.