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C5914

Sigma-Aldrich

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

Sinonimo/i:

Dissociation Solution

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100 ML
74,30 €

74,30 €


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Cambia visualizzazione
100 ML
74,30 €

About This Item

Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.75

74,30 €


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Livello qualitativo

Sterilità

sterile-filtered

Nome Commerciale

BioReagent

Stato

liquid

Concentrazione

1 ×

tecniche

cell culture | mammalian: suitable

pH

6.9-7.1

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Descrizione generale

Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.[1]

Applicazioni

Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture,[2] U87 glioblastoma tumor cells[3] and cardiac stem cells (CSCs).[4]
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
Kevin C Osum et al.
Scientific reports, 8(1), 8295-8295 (2018-05-31)
Type 1 diabetes is caused by autoreactive T cell-mediated β cell destruction. Even though co-inhibitory receptor programmed death-1 (PD-1) restrains autoimmunity, the expression and regulation of its cognate ligands on β cell remains unknown. Here, we interrogated β cell-intrinsic programmed

Domande

1–5 di 5 domande  
  1. What is the Department of Transportation shipping information for this product?

    1 risposta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  2. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    1 risposta
    1. We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

      Utile?

  3. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    1 risposta
    1. It will work with most cell types.

      Utile?

  4. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    1 risposta
    1. Yes, it does contain EDTA; the concentration is proprietary.

      Utile?

  5. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    1 risposta
    1. No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

      Utile?

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